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Meeting ID: 953 8784 0890
Super-resolution imaging of complex materials: chromatography and (extra)cellular nutrients
Lydia Kisley, Warren E. Rupp Assistant Professor, Case Western Reserve University, Departments of Physics and Chemistry
Abstract: Single-molecule spectroscopy and super-resolution fluorescence microscopy have become seminal tools for scientists due to their ability to resolve heterogeneity normally obscured in traditional ensemble measurements. Single-molecule spectroscopy has enabled important findings in areas such as cellular biophysics and catalysis, yet, single-molecule techniques have had limited use in the study of materials. From a macroscale engineering perspective, many materials are optimized empirically to decide what conditions work “best,” resulting in little understanding of the physics behind why the selected conditions perform the way they do. On the other hand, single-molecule studies of materials have focused on model, fundamental systems: materials simplified to have only a few components so they can be well-described by statistical models, but far from conditions for their intended use. Our lab’s goal is to advance the single-molecule materials field towards more complex conditions relevant to industrial and medical questions. In this talk I will present our work 1) studying chromatographic separation stationary phases used in industrial columns for separating chiral molecules with three-dimensional single-molecule imaging and 2) developing a super-resolution expansion microscopy method to sense small-molecule cellular nutrients in the extracellular environment. Overall, our single-molecule approach will allow us to study materials that are realistic, multi-component, and complex to connect fundamental molecular observations to materials challenges in industry and medicine.