College of Arts and Sciences

Reconstructing cell phenotypic transition dynamics from single cell data

Recent advances in single-cell techniques catalyze an emerging field of studying how cells convert from one phenotype to another, i.e., cell phenotypic transitions (CPTs). Two grand technical challenges, however, impede further development of the field. Fixed cell-based approaches can provide snapshots of high-dimensional expression profiles but have fundamental limits on revealing temporal information, and fluorescence-based live cell imaging approaches provide temporal information but are technically challenging for multiplex long- term imaging. My lab is tackling these grand challenges from two directions, with the ultimate goal of integrating the two directions to reconstruct the spatial-temporal dynamics of CPTs.

Continue reading… Jianhua Xing (University of Pittsburgh)

On the quest of finding the surface of articular cartilage

The primary role of articular cartilage (AC) is to provide a smooth lubricated surface between contacting and moving bones, which allows for ultralow friction as well as wear protection to the sliding epiphysis for almost a century in healthy people. The physical and chemical nature of the topmost surface of AC has intrigued researchers since it was first reported in 1951, called the “lamina splendens”. This layer has been the source of heated and controversial scientific debate since it was first reported. The lamina splendens is important because it forms the interfaces between the cartilage and synovial fluid,

Continue reading… Roberto Carlos Andresen Eguiluz (UC Merced)

Title:  Protein dynamics: Connecting in vitro, in cell, and in vivo

Although biomolecules evolved to function in the cell, most biochemical and biophysical studies have been carried out in vitro. A combination of in vitro, in-cell, and in vivo studies will highlight how steric and non-steric interactions modulate protein folding and protein-RNA interactions. I will introduce a customized pipeline that combines meganuclease mediated transformation with fluorescence-detected temperature-jump microscopy to image fast dynamics of biomolecules in living zebrafish with single-cell resolution. To interpret in vivo and in-cell results, an in vitro systematic series of solvation environments will distinguish contributions from non-steric and steric interactions to stability,

Continue reading… Caitlin Davis (Yale University)